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DOT Urine Drug Testing:

What is this test?

DOT urine drug testing is a federally regulated drug screening required for employees who perform safety-sensitive functions under the U.S. Department of Transportation (DOT).

Testing follows strict guidelines established by 49 CFR Part 40 and includes identity verification, chain-of-custody procedures, and laboratory analysis by certified laboratories.

Who This test is for

DOT drug testing is required for individuals working in safety-sensitive positions regulated by:

  • FMCSA (Federal Motor Carrier Safety Administration

  • FAA (Federal Aviation Administration)

  • FTA (Federal Transit Administration)

  • FRA (Federal Railroad Administration)

  • PHMSA (Pipeline & Hazardous Materials Safety Administration)

  • Transportation employers subject to DOT regulations

  • Employers enrolled in DOT testing programs or consortia

DOT testing is not optional and must follow federal procedures exactly

How the Process works

  1. Appointment is scheduled through our booking system

  2. Donor presents valid government-issued photo identification

  3. Specimen is collected following DOT chain-of-custody procedures

  4. Sample is sent to a DOT-certified laboratory for analysis

  5. Results are reviewed by a Medical Review Officer (MRO)

  6. Results are sent to DER

How to Prepare

  • Bring a valid government-issued photo ID

  • Arrive on time for your scheduled appointment

  • Be prepared to provide a urine specimen during the visit

  • access to water will be available (up to 40 oz only for testing)

  • we will Inform your employer if you are unable to produce a specimen

What to expect at your appointment

  • Appointments typically take 15–20 minutes. Identity verification is required, and the collection process is conducted professionally and confidentially in accordance with DOT regulations.

    Observed collections may be required in certain circumstances, as determined by DOT rules.

  • You will receive a copy of of the cCF form

How to book

  • DOT urine drug testing appointments must be scheduled in advance.

Schedule DOT Testing